Maria Grillet, Instituto de Ecologia y Zoologia Tropical, Universidad Central de Venezuela
3 February 2014
In our recent publication (Convit et al. 2013, 6:289), we provided an incorrect citation describing the use of the polymerase chain reaction (PCR) employing Onchocerca volvulus-specific DNA probes to examine pools of flies (see page 5). The correct reference is number 37 (see list of references, page 13) or reference 1 (below). Additionally, we did not report that during 2012, the flies were not prepared using the conventional silica method described in these references but instead used a new methodology, oligonucleotide magnetic bead capture, which was recently published (see 2, below). Therefore, our study becomes the first one in the onchocerciasis endemic region of the Americas that uses and validates this new methodology to detect O. volvulus infection in pools (now 200 females) of wild flies.
References
1. Rodríguez-Pérez MA, Domínguez-Vásquez A, Unnasch TR, Hassan HK, Arredondo-Jimenez JI, Orozco-Algarra ME, Rodríguez-Morales KB, Rodríguez-Luna IC, Prado-Velasco FG: Interruption of transmission of Onchocerca volvulus in the Southern Chiapas focus, Mexico. PLoS Negl Trop Dis 2013, 7: e2133
2. Gopal H, Hassan HK, Rodríguez-Pérez MA, Toé LD, Lustigman S, Unnasch TR: Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies. PLoS Negl Trop Dis 2012, 6:e1712.
Two minor corrections: Convit et al. 2013, 6:289
3 February 2014
In our recent publication (Convit et al. 2013, 6:289), we provided an incorrect citation describing the use of the polymerase chain reaction (PCR) employing Onchocerca volvulus-specific DNA probes to examine pools of flies (see page 5). The correct reference is number 37 (see list of references, page 13) or reference 1 (below). Additionally, we did not report that during 2012, the flies were not prepared using the conventional silica method described in these references but instead used a new methodology, oligonucleotide magnetic bead capture, which was recently published (see 2, below). Therefore, our study becomes the first one in the onchocerciasis endemic region of the Americas that uses and validates this new methodology to detect O. volvulus infection in pools (now 200 females) of wild flies.
References
1. Rodríguez-Pérez MA, Domínguez-Vásquez A, Unnasch TR, Hassan HK, Arredondo-Jimenez JI, Orozco-Algarra ME, Rodríguez-Morales KB, Rodríguez-Luna IC, Prado-Velasco FG: Interruption of transmission of Onchocerca volvulus in the Southern Chiapas focus, Mexico. PLoS Negl Trop Dis 2013, 7: e2133
2. Gopal H, Hassan HK, Rodríguez-Pérez MA, Toé LD, Lustigman S, Unnasch TR: Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies. PLoS Negl Trop Dis 2012, 6:e1712.
Competing interests
I declare that I have no competing interests.