Table 1 Sequences of oligonucleotide primers used for real-time PCR
Target | Forward primer (5′-3′) | Reverse primer (5′-3′) | Length(bp) |
|---|---|---|---|
TNF-a | GAACTTCGGGGTGATCGGTC | TGTCTTTGAGATCCATGCCG | 221 |
IL-12p40 | TGCCAGGAGGATGTCACCT | GGCGGGTCTGGTTTGATGAT | 137 |
iNOS | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGTCAC | 127 |
Arginase-1 | CTCCAAGCCAAAGTCCTTAGAG | AGGAGCTATCATTAGGGACATC | 185 |
Ym1 | AGAAGGGAGTTTCAA ACCTGGT | GTCTTGCTCATGTGTGTAAGTGA | 109 |
GAPDH | CAACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACAC-3′ | 224 |