Increased expression of CD25, CD83, and CD86, and secretion of IL-12, IL-23, and IL-10 by human dendritic cells incubated in the presence of Toll-like receptor 2 ligands and Giardia duodenalis

Table 1 DC activation markers expressed by human DCs incubated in the presence of ligands for TLR4, TLR2/TLR1, or TLR2/TLR6, and in the presence or absence of G. duodenalis lysate

Molecule	LPS	LPS + Gd	Pam3CSK	Pam3CSK + Gd	Pam2CSK4	Pam2CSK4 + Gd
CD25	84 [73, 90]^#	82 [66, 88]*	8 [3, 21]	20 [7, 30]*	10 [3, 27]	21 [9, 35]*
CD83	90 [85, 95]	84 [80, 93]*	18 [12, 51]	35 [22, 59]*	23 [15, 51]	39 [24, 63]*
CD86	98 [97, 99]	96 [93, 97]*	51 [48, 61]	67 [59, 79]*	56 [48, 71]	70 [61, 86]*
HLA-DR	99 [98, 99]	97 [95, 98]*	88 [84, 97]	90 [86, 97]	88 [84, 96]	92 [84, 97]

^#Median percentage of positive cells (25% and 75% percentiles given in square brackets) obtained with cells from ten donors, except for CD86, which could be analyzed for eight donors only (data of isotype controls were subtracted).
*p < 0.05 compared to DCs incubated in the presence of LPS, Pam3CSK, or Pam2CSK4 but in the absence of G. duodenalis (Wilcoxon matched-pairs signed rank test).

ISSN: 1756-3305