Figure 4

A. aegypti SGE induces lymphocyte apoptosis and cleavage of pro-caspase 3 and pro-caspase-8. Total spleen cells were incubated with Con A in medium only or in presence of A. aegypti SGE for 4 h. Annexin V staining was evaluated by flow cytometry in total cells, CD4⁺, CD8⁺ and CD19⁺ cells from WT mice (A) or CD4⁺ T cells from Bim^+/− and Bim^−/− mice (C). Lysates of similar cell cultures were blotted against a monoclonal antibody against pro-caspase-3 and pro-caspase-8 (B).