Figure 5

Memory cells are resistant to A. aegypti SGE effects. Spleen cells from non-sensitized (A) and A. aegypti-sensitized BALB/c mice (B) were incubated with medium only or with A. aegypti SGE (5 μg/mL) and/or stimulated with Con A (0.5 μg/mL) for 72 h. Cells from An. aquasalis-sensitized mice were cultured with medium only or in the presence of 5 μg/mL An. aquasalis SGE (An.) and/or 5 μg/mL A. aegypti SGE (Ae.) and then stimulated with 0.5 μg/mL Con A (C). Non-adherent DO11.10 spleen cells were adoptively transferred to BALB/c mice and after 7 days, recipient mice were sensitized with OVA and complete Freund’s adjuvant (40 μg/animal). Spleen cells from non-sensitized (D) and sensitized mice (E) were obtained after 7 days and cultured in the presence of medium or A. aegypti SGE and stimulated with Con A (0.5 μg/mL) or OVA (100 μg/mL). Phenotype of naïve cells (CD62L^HIGH/CD44^LOW), T_EM subset (CD62L^LOW and CD44^HIGH) and T_CM subset (CD62L^HIGH and CD44^HIGH) from non-sensitized (F) or sensitized mice (G) were evaluated by flow cytometry after 72 h cultured in presence of medium or A. aegypti SGE and stimulated with Con A or OVA. ^*p < 0.05 versus respective control group.