Figure 3

Phylogenetic network (NeighborNet) constructed by Splitstree for the 128 L. infantum strains studied. The NeighborNet algorithm detected the same population substructures inferred by STRUCTURE (Figure 2, Table 1) for population 3 (Sub3A, Sub3B¹ and Sub3B²) and population 4 (Sub4A, Sub4B, Sub4C¹ and Sub4C²). Subdivision of populations 1 and 2 is not supported by NeighborNet analysis. TR, Turkey; CY, Cyprus; BG, Bulgaria; GR, Greece; AL, Albania; HR, Croatia; FR, France; ES, Spain; PT, Portugal; MON-1, L. infantum zymodeme MON-1; MON-98, L. infantum zymodeme MON-98. Strains that presented identical MLM Types (Table 1) are underlined. Strains are represented by coloured boxes that correspond to their population or subpopulation assignment according to STRUCTURE analysis (Figure 2).