Differential expression of primary transcripts of Toxoplasma -upregulated mature miRNAs in human macrophage. Human macrophage were exposed to Toxoplasma for 6 h, 12 h and 24 h and primary transcripts (pri-miRNAs) of select miRNAs were quantified by qRT-PCR. The amount of pri-miRNAs was obtained by normalizing to the level of GAPDH in the samples. Data are expressed as the amount of pri-miRNAs in the infected samples relative to the control uninfected samples and representative of three independent experiments. *, p,0.05 vs. the non-infected control.