Development of an in vitro drug sensitivity assay for Trichuris muris first-stage larvae

Parasites & Vectors

Table 1 Comparison between the drug sensitivity motility assay for T. muris L1 and adult parasites

	*T. muris* L1 assay	Adult *T. muris* assay
Duration of drug assay^a	24 hours	72 hours
Number of parasites per well	20 to 200	2 to 4
Dissection of animal host required	No	Yes
Subjective analysis	Yes	Yes
Viability marker alternative	No	Yes^b
Total operational hours	3.5 hours^c	6.5 hours^d
Applicability to high-throughput screening	No	No

^a: Time between start of the assay and its read-out. ^b: Resazurin (Alamar Blue^®). ^c: Including preparation and reading of the assay. ^d: Including Infection, keeping and dissection of the animal host, collection of the parasites, preparation and reading of the assay.

ISSN: 1756-3305