Figure 2
The identification of recombinant plasmids and in vitro expression of compound genes. A: Identification of recombinant plasmids pSAG1; pSAG1/SAG3; pSAG1/SAG3-CTXA2/B. Lane M1: DNA Marker 6000 Lane1. pSAG1 digested by EcoR I and BamH I; Lane2. pSAG1/SAG3 digested by EcoR I and Kpn I; Lane3. pSAG1/SAG3-CTXA2/B digested by EcoR I and Hind III; Lane 4. PCR product of SAG1; Lane 5. PCR product of SAG3; Lane 6. PCR product of CTXA2/B; Lane M2: DNA Marker 5000. B: RT-PCR results of Hela cell transfected by recombinant plasmid. Lane 1, 2, 3, 4, 5: Hela cells transfected by Liposome, pcDNA3.1(−), pSAG1, pSAG1/SAG3, pSAG1/SAG3-CTXA2/B respectively; Lane M DNA Marker 2000.