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Table 1 Measurement of the cytokines in the immunized mice by sandwich ELISA

From: Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii

Immunization regimena

Cytokine production (pg/ml)b

SIc

 

IFN-γ

IL-4

 

PBS

11±5

N.D

0.17

pcDNA3.1

13±4

N.D

0.32

pSAG1

24±6

N.D

1.38

pSAG1/SAG3

47±7

N.D

2.47

pSAG1/SAG3-CTXA2/B

89±10

N.D

4.28

  1. a Mice were immunized by i.m. route on day 0 and day 14 and day 28 with PBS, pcDNA3.1, pSAG1, pSAG1/SAG3, pSAG1/SAG3-CTXA2/B.
  2. b The splenocytes taken from mice (n = 3, each group) 2 weeks after the last immunization were examined for cytokine production by sandwich ELISA. Values for IFN-γ from 96 h, values for IL-4 from 24 h. N.D means not detectable.
  3. c The results of proliferation assays are expressed as the stimulation index (SI), calculated as the ratio between the mean counts per minute (cpm) for triplicate stimulated cultures and the mean counts per minute for triplicate unstimulated cultures. SI values 2.5-fold greater than the SI of the control groups were considered as significant.
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Parasites & Vectors

ISSN: 1756-3305

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