ELISA standard curve for β-glucanase. Decreasing concentrations of synthetic β-glucanase were titrated against 1:5000 dilution of the polyclonal anti-glucanase antibody to generate ELISA standard curves such as the one shown here. These curves were used to quantify recombinant β-glucanase expression from each R. rhodnii clone. All points represent the mean with standard error bars of triplicate samples. Experiments were performed at least twice.