A) Levels of total IgG anti-TrV antibodies, B) Levels of total IgG1 anti-TrV antibodies, C) Levels of total IgG2a anti-TrV antibodies elicited in mice BALB/c inoculated with empty TrV particles (eTrV) and RNA-full TrV capsids (TrV); D) ratio IgG2a/IgG1 antibodies. Serum samples were taken on days 45 after injection. Each column represents the mean with standard deviation (SD) from the ratio of optical density (OD490 nm) and cut-off values (three mice per group). Cut-off value was defined as: mean + 3SD. Abbreviations: IP: intraperitoneal route; PAb: Polyclonal antibodies.