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Table 4 Results of Luminex assay and real time PCR with 15 DNA from stool samples

From: LUMINEX®: a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools

  E.dispar E. hist E. moshk E hart E.coli Real time PCR**
CTL+ Eh_ 1* −41 981 93 140 −89 -
CTL+ Ed_1 1368 54 −61 214 −79 -
CTL+ Em_ 2 −76 −48 547 −45 −86 -
CTL+ Ehart_1 −82 −87 −80 1844 −85 -
CTL+ Ecoli −75 −60 −84 −53 1194 -
Sample 1 530 −15 −64 181 −73 E.dispar
Sample 2 772 −10 −74 728 −76 E.dispar
Sample 3 −77 −76 −71 −79 −68 Negative
Sample 4 −44 1094 135 201 59 E. hist
Sample 5 −43 1144 154 185 75 E. hist
Sample 6 597 −4 −75 578 −80 E.dispar
Sample 7 −79 −70 −77 −89 −87 Negative
Sample 8 −50 1027 101 172 44 E. hist
Sample 9 −39 1144 117 197 49 E. hist
Sample10 −39 1145 172 255 97 E. hist
Sample 11 −85 −69 −86 −94 −93 Negative
Sample 12 −83 −84 −91 −95 −88 Negative
Sample 13 487 −23 −78 394 −73 E.dispar
Sample 14 −75 −69 −81 −78 −71 Negative
Sample 15 530 −11 −65 478 −63 E.dispar
Average blank*** 111 101 116 117 115  
  1. *CTL= positive control from cloned-derived PCR biotinylated products.
  2. **Real time PCR specific for E. histolytica and E. dispar.
  3. *** From triplicate.
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