Figure 2

Sequence comparison by pairwise alignment and a phylogenetic tree. a) ClustalOmega pairwise alignment of Strongylus vulgaris SXP (SvSXP) with homologues in other species. SvSXP (accession number: KC155360) is compared with Cylicostephanus goldi SXP partial homologue, Necator americanus surface-associated antigen (Na-SAA) (ACE79378.1), Ancylostoma caninum immunodominant hypodermal antigen (Ac16) (ABD98404.1), Ascaris suum 14 kDa antigen (As14) (BAB67769.1), Ascaris lumbricoides antigen 1(Ag1) (ADB45853.1) and Parascaris equorum partial homologue. The signal peptide for each sequence is underlined. Asterisks (*) denote identical amino acids, double dots (:) denote conserved amino acid changes and a single dot (.) denotes semi-conserved amino acid changes. Hyphen (−) indicates that a space was inserted in the sequence to improve alignment. The percent similarity to SvSxP is listed for each homologous protein. b) Phylogenetic tree of the above mentioned sequences.