Table 4 Comparison of PrioCHECK ® Toxoplasma Ab SR ELISA-results (cut-off PP 20) from serum samples of 300 sheep from Austria (Groups 3 and 4) to results obtained by IFAT (cut-offs 1:40 and 1:80) and IHA and inter-rater agreement (kappa value)
IFAT serum cut-off 1:40 | IFAT serum cut-off 1:80 | IHA serum cut-off 1:80 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
positive | negative | total | positive | negative | total | positive | negative | total | ||
ELISA serum | positive | 139 | 2 | 141 | 135 | 6 | 141 | 138 | 3 | 141 |
negative | 11 | 148 | 159 | 5 | 154 | 159 | 3 | 156 | 159 | |
total | 150 | 150 | 300 | 140 | 160 | 300 | 141 | 159 | 300 | |
Kappa | 0.913 | 0.926 | 0.960 | |||||||
Standard Error | 0.0235 | 0.0218 | 0.0162 | |||||||
95% CI | 0.867-0.959 | 0.884-0.969 | 0.928-0.992 | |||||||