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Table 4 Comparison of PrioCHECK ® Toxoplasma Ab SR ELISA-results (cut-off PP 20) from serum samples of 300 sheep from Austria (Groups 3 and 4) to results obtained by IFAT (cut-offs 1:40 and 1:80) and IHA and inter-rater agreement (kappa value)

From: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep

   IFAT serum cut-off 1:40 IFAT serum cut-off 1:80 IHA serum cut-off 1:80
   positive negative total positive negative total positive negative total
ELISA serum positive 139 2 141 135 6 141 138 3 141
  negative 11 148 159 5 154 159 3 156 159
  total 150 150 300 140 160 300 141 159 300
  Kappa 0.913    0.926    0.960   
  Standard Error 0.0235    0.0218    0.0162   
  95% CI 0.867-0.959    0.884-0.969    0.928-0.992