Table 2 Primer sequences of the tested vector-borne pathogens (genera or species)
From: Feline vector-borne pathogens in the north and centre of Portugal
Agents | Amplified region | Primer forward (5′-3′) | Primer reverse (5′-3′) | Final [primer] (μM) |
|---|---|---|---|---|
Anaplasma/Ehrlichia spp. | 16S rRNA | GGGTGAGTAATGCRTAGGAATCTACCTAGTA | GGATTATACAGTATTACCCAYCATTTCTARTG | 0.5 |
Babesia spp. | 18S rRNA | GTGGCTTTTCCGATTCGTCG | TTCCTTTAAGTGATAAGGTTCACAAAACTT | 0.3 |
Babesia canis | 18S rRNA | CGGTTTGACCATTTGGTTGGTTA | CCATGCTGAAGTATTCAAGACAAAAGT | 0.3 |
Babesia vogeli | 18S rRNA | CATTCGTTTGGCTTTTTCGAG | CCATGCTGAAGTATTCAAGACAAAAGT | 0.3 |
Hepatozoon felis | 18S rRNA | CTTACCGTGGCAGTGACGGT | TGTTATTTCTTGTCACTACCTCTCTTATGC | 0.3 |
Leishmania infantum | kinetoplast DNA | AACTTTTCTGGTCCTCCGGGTAG | ACCCCCAGTTTCCCGCC | 0.9 |
Rickettsia spp. | 16S rRNA | AGCCTGATCCAGCAATACCGA | CGGGGCTTTTTCTGCAAGTAA | 0.3 |