Figure 4From: Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceumRespiratory burst activity in D. pseudospathaceum exposed sticklebacks. Head kidney leukocytes were stimulated with zymosan (A) and D. pseudospathaceum antigens (B). Respiratory burst activity was recorded with a chemiluminescence assay (RLUarea) 1.5 and 5 days after the last exposure (mean and SE, 0/0 = sham exposed controls, 0/1 = single exposure only, 5/0 = pre-exposures only, 5/1 = pre- and single exposures, different letters above bars indicate significant differences p < 0.05 with sequential Bonferroni correction for multiple testing). Note: different y-axis scaling in A and B.Back to article page