Table 4 Diagnostic tools used by veterinarians to confirm a suspected case of CanL
|  | France n = 994 | Greece n = 201 | Italy n = 231 | ||||||
|---|---|---|---|---|---|---|---|---|---|
Technique % | Never | Occasional to frequent | Always | Never | Occasional to frequent | Always | Never | Occasional to frequent | Always |
Cytology | |||||||||
Lymph nodes | 34.6 | 52.8 | 12.6 | 21.4 | 58.6 | 20 | 46.7 | 48.0 | 5.3 |
Bone Marrow | 65.5 | 30.6 | 3.9 | 69.6 | 29.4 | 1.1 | 59.9 | 33.6 | 6.6 |
Skin lesions | 65 | 28.9 | 6.1 | 56.6 | 40.4 | 3 | 28.8 | 56.5 | 14.7 |
Serology | |||||||||
IFAT | 22.7 | 28.7 | 48.6 | 16 | 45 | 39 | 4.3 | 48.9 | 46.8 |
Rapid tests | 42 | 33.9 | 24 | 4.6 | 37.1 | 58.3 | 30.9 | 38.2 | 30.9 |
ELISA | 13.5 | 34.7 | 51.9 | 14.3 | 58.9 | 26.8 | 25.2 | 37.7 | 37.1 |
Other techniques | |||||||||
PCR | 55.7 | 36.6 | 7.7 | 52.1 | 46.4 | 1.4 | 20.9 | 66.9 | 12.2 |
Protein electrophoresis | 42.3 | 41 | 16.8 | 76.6 | 20.4 | 2.1 | 10.6 | 39.8 | 49.7 |
Histopathology | 51.1 | 45.8 | 3.1 | 72.3 | 27.7 | 0 | 64.9 | 35.1 | 0 |
Immunohistochemistry | 94.3 | 4.7 | 1 | 94.5 | 5.5 | 0 | 83 | 13.8 | 3.2 |
|  | Slovenia n = 49 | Spain n = 483 | Portugal n = 141 | ||||||
Cytology | |||||||||
Lymph nodes | 2 | 4 | 0 | 14.3 | 80.2 | 5.5 | 19.4 | 72.1 | 8.6 |
Bone Marrow | 2 | 2 | 0 | 18.1 | 71.4 | 10.5 | 36.2 | 53.2 | 10.6 |
Skin lesions | 6.1 | 2 | 0 | 34.2 | 61.8 | 3.9 | 82.9 | 14.6 | 2.4 |
Serology | |||||||||
IFAT | 0 | 4 | 0 | 6.3 | 37.9 | 55.9 | 9.4 | 36.7 | 25.9 |
Rapid test | 0 | 2 | 2 | 11.5 | 54.0 | 34.5 | 20 | 43.5 | 36.5 |
ELISA | 0 | 2 | 6.1 | 42.3 | 47.3 | 10.4 | 43.9 | 43.9 | 12.2 |
Other techniques | |||||||||
PCR | 0 | 2 | 2 | 8.3 | 86.6 | 5.1 | 57.8 | 37.6 | 4.7 |
Protein electrophoresis | 0 | 2 | 0 | nd | Nd | nd | 96.5 | 1.8 | 1.8 |
Histopathology | 0 | 4.1 | 0 | 35.3 | 63.9 | 0.8 | 91.5 | 6.8 | 1.7 |
Immunohistochemistry | 0 | 2 | 0 | 15.0 | 40.4 | 44.6 | 33.8 | 48.8 | 17.5 |