Serological diagnosis of canine leishmaniosis: comparison of three commercial ELISA tests (Leiscan®, ID Screen® and Leishmania 96®), a rapid test (Speed Leish K®) and an in-house IFAT

Table 1 Results of measures of diagnostic performance of serological tests studied based on manufacturer’s recommendations and based on ROC cut-off values

Measures of diagnostic performance
	ID screen^®a	ID screen^®b	Leiscan^®a	Leiscan^®b	Leishmania 96^®a	Leishmania 96^®b	IFAT^a	IFAT^b	Speed Leish K^®
Sensitivity	0.953	0.963	0.925	0.953	0.925	0.832	0.869	0.813	0.636
Specificity	1.000	1.000	1.000	1.000	0.896	0.980	0.917	0.990	1.000
Accuracy	0.975	0.980	0.961	0.975	0.911	0.902	0.892	0.897	0.808
Kappa index	0.951	0.961	0.921	0.951	0.822	0.804	0.783	0.795	0.622
Youden index	0.953	0.963	0.925	0.953	0.821	0.811	0.786	0.803	0.636

^aMeasures of diagnostic performance based on manufacturer’s recommendations.
^bMeasures of diagnostic performance based on ROC cut-off values. The new cut-off established for each test was: ID Screen^® (Positive ≥ Ratio 41.97; Negative < Ratio 41.97), Leiscan^® (Positive ≥ Ratio 0.77; Negative < Ratio 0.77), Leishmania 96^® (Positive ≥ Ratio 0.52; Negative < Ratio 0.52) and IFAT (Positive ≥1:160; Negative <1:160).

ISSN: 1756-3305