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Table 2 Sensitivity of egg identification in two-species pools of ten eggs in different mix ratios

From: Rapid protein profiling facilitates surveillance of invasive mosquito species

Mix ratio

10/0

9/1

8/2

7/3

6/4

5/5

Number of pools

24

24

24

24

24

12

Total number of spots measured (technical replicates)

96

96

96

96

96

48

Number of the 4 technical replicates yielding identification of both species

 

4

0.0%

0.0%

0.0%

70.3%

75.0%

91.7%

≥3

0.0%

0.0%

16.7%

91.7%

91.7%

100.0%

≥2

0.0%

12.5%

20.8%

100.0%

100.0%

100.0%

≥1

0.0%

25.0%

37.5%

100.0%

100.0%

100.0%

  1. Three biological replicates have been performed per pools containing Ae. aegypti/Ae. japonicus, Ae. albopictus/Ae. geniculatus, Ae. albopictus/Ae. japonicus, Ae. geniculatus/Ae. japonicus.