Figure 1
PCR amplicons from the mitochondrial genome of the horse louse, Haematopinus asini . (A) Amplicons generated with the horse-louse-specific primers, 12sB2448F–12sB2448R (lane 2), 16sB2448F–16sB2448R (lane 3), cox1B2448F–cox1B2448R (lane 4) and cox2B2448F–cox2B2448R (lane 5) from four mitochondrial minichromosomes. Lane 1 and lane 6: 100-bp Ladder and 1-kb Ladder (BioSciences). (B) Amplicons generated with the primer pair B2448F-B2448R from the coding regions of all of the mitochondrial minichromosomes of the horse louse (lane 2). Lane 1: 500-bp DNA Ladder (Tiangen). (C) PCR verification of the mt minichromosomes of the horse louse. Lane 1 and 12: 100-bp ladder. Lane 2 and 13: 1-kb ladder. Lane 3–11: PCR amplicons from the nine minichromosomes of the horse louse: K- nad4 -atp8-atp6-N, nad2 -I-cox1-L 2 , D-Y- cox2 -S 1 -S 2 -P-cox3-A, E- cob -V, Q-nad1-T-G- nad3 -W, H- nad5 -F-nad6, M, L 1 -rrnL and R-nad4L- rrnS -C. Genes from which PCR primers were designed are in bold.