Table 1 The thermal profile for each polymerase chain reaction, with the used primers

TrF and TrR2^a

sno-RNA-Cl1 T. rangeli

95°C/5 min; 15 cycles of 95°C/30 s, 63°C/1 min, 72°C/30 s, 20 cycles of 95°C/30 s, 60°C/1 min, 72°C/30 s and 72°C/5 min.

TC, TC1 and TC2^b

Mini-exon gene T. cruzi

94°C/5 min, 61°C/30 s, 72°C/30 s, 15 cycles of 94°C/1 min, 61°C/30 s, 72°C/30 s and 94°C/1 min, 61°C/30 s, 72°C/10 min.