Table 2 Microsatellite markers used to estimate population structure in R. microplus ticks from southern Texas

PNC75

64

2

1/40

NED

4

139, 143, 145, 147

[28]

PNC98

55

3

1/10

NED

4

135, 139, 141, 143

[28]

PNC153

48

na^a

1/15

VIC

16

132, 136, 140, 144, 148, 152, 156, 160, 164, 172, 176, 180, 184, 188, 192, 196

[28] but redesigned R primer (PNC153-R2): TTCAAAGTTCATATGCATGGTC

BmC07

57

1

1/100

NED

8

132, 142, 158, 170, 172, 178, 190, 192

[29] but redesigned R primer (BmC07-R2); GTCAGCCATATGTTCAACCAGA

BmD10

57

1

1/100

6FAM

2

152, 154

[29]

BmB12

67

na^a

1/60

6FAM

2

297, 301

[29] but redesigned R primer (BmB12-R2): CGTATGAAGCTATGATGAATAGGAGACGTG

LTF4.3

48

na^a

1/15

PET

3

295, 297, 319

[30]

SJB411

55

3

1/10

6FAM

19

159, 187, 191, 195, 215, 219, 223, 227, 231, 239, 243, 251, 259, 263, 267, 271, 275, 279, 283

[30]

KRGinv

55

na

na

VIC

20

138, 142, 146, 150, 154, 158, 162, 166, 170, 174, 210, 246, 252, 256, 258, 262, 266, 270, 274, 278

[30] Removed from study.

ATC12

64

2

1/40

PET

12

137, 143, 155, 158, 161, 164, 167, 170, 173, 176, 179, 182

[26] F-CAAGCACAGGACCGAGTTGA

[26] R-GTGTGCTTTCGCAATGATCG

ATC15

56

4

1/30

PET

4

187, 205, 208, 211

[26] F-AAAGATTCATGAAGGATGTTGATCG

[26] R-GCCTACAAATTCAACTGAGGGAAAA

ATT20

56

4

1/30

6FAM

25

190, 226, 229, 232, 235, 238, 241, 244, 247, 250, 253, 256, 259, 262, 265, 268, 271, 274, 277, 280, 283, 286, 289, 292, 295

[26] F-CGGTTAATCTACAAACGAAGTCTTG

[26] R-TTTTTATGTAGTGCTTTTTCAACTTTCA