Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae)

Table 2 Mean survival rates of Aedes aegypti released into rooms containing black cloths impregnated with fungal conidia using three different carriers

Survival (%)
Time since cloths first placed in rooms (days)	Conidia + T	Conidia + V	Conidia + V + I
0–5	38 ± 1 b	36.6 ± 1.5 b	32.6 ± 2.08 d
6-11	49.3 ± 2.51 b	50 ± 2.64 b	40.6 ± 0.57d
12 -17	81 ± 2.08 a	78.6 ± 2.80 a	60 ± 2.64 c
18-23	82 ± 1 a	81.3 ± 3.21 a	64.6 ± 2.51 bc
24-29	ND	82.6 ± 1.52 a	77.3 ± 0.57 ab
30-35	ND	ND	83.3 ± 2.51 a
Control	83.3 ± 2.51 a	84.6 ± 2.08 a	87.3 ± 4.16 a

Three fungal treatments were tested: conidia + Tween (T), conidia + vegetable oil (V) and conidia + vegetable oil + isoparaffin (I).
At the end of each time period, the surviving mosquitoes were captured and a new cohort released into the room. Mean survival values followed by the same letter indicate that the results for each treatment were not statistically different when using a one way analysis of variance followed by Tukey’s test (5% probability) to compare the different periods that cloths had been left in the rooms. ND = not determined. Control results for each time period were not significantly different for each repetition (P > 0.01) and were thus pooled and shown as a single mean value for each treatment.

ISSN: 1756-3305