Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia

Table 1 Molecular identification assays published for the Obsoletus group in the Palaearctic region and used during the ring trials

Reference	Identified species	Molecular marker used¹	Type of assay
[39]	C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi	COI	Species-specific multiplex PCR
[37]	C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi, C. montanus	ITS1	Species-specific multiplex PCR
[37, 44]	C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi	ITS2/ITS1	Species-specific multiplex PCR
[36]	C. obsoletus, C. scoticus, C. chiopterus, C. dewulfi	ITS1	Microarray

¹COI : Cytochrome Oxidase 1, ITS1 : Internal Transcribed Spacer 1, ITS2 : Internal Transcribed Spacer 2.

ISSN: 1756-3305