Ultrastructural immunolocalization in leg apodemes of 1-d-old mosquitoes. CPF3 (left panels) and CPLCG3/4 (right panels) are shown in the flexor tibiae apodeme cuticle (A, B); and extensor tibiae apodeme (C, D). (E, F) Control sections treated with the appropriate pre-immune serum; note the granulated structures in the endocuticle. Sections were incubated with goat anti-mouse (A, C, E) or with goat anti-rabbit (B, D, F) secondary antibodies conjugated to 10- and 5-nm gold particles, respectively. ex, exocuticle; en, endocuticle Scale bar=500 nm.