Infection with dengue-2 virus alters proteins in naturally expectorated saliva of Aedes aegypti mosquitoes

Parasites & Vectors

Table 1 Identified proteins with accession numbers and fold change information

*Ae. aegypti* salivary proteins identified by mass spectrometry with fold change due to DENV infection
GenBank ID	Protein name	Spot	Spot fold change
gi\|108875535\|	DEAD-Box ATP-Dependent RNA Helicase^‡	1	−1.5
gi\|108877982\|	Beta Chain Spectrin^‡	2	+1.0
gi\|157109431\|	Hypothetical Secreted Protein AAEL000748^‡	3	−4.0
gi\|108878609\|	Adenosine Deaminase	4	−2.8*
gi\|18568326\|	Putative Adenosine Deaminase	5	−5.2*
gi\|1094353\|	Apyrase^‡	6	−1.1
gi\|108877845\|	Putative Apyrase^‡	7	+1.1
gi\|108877687\|	Inosine-Uridine Preferring Nucleoside Hydrolase	8	−7.9*
gi\|18568280\|	Putative Purine Hydrolase	9	−3.8*
gi\|94468358\|	Salivary Anti-FXa Serpin	10	−4.8*
gi\|157109433\|	Hypothetical Protein AAEL000732	11	−7.7*
gi\|157131306\|	Putative Serine Protease Inhibitor (Serpin-4)	12	−19.4*
gi\|94468352\|	Angiopoietin-Like Protein Variant^‡	13	−1.8
gi\|94468642\|	Putative 34kD Family Secreted Salivary Protein^‡	14	−1.3
gi\|222447044\|	D7^‡	15	−4.5
gi\|222447044\|	D7^‡	16	−1.3
gi\|222447044\|	D7^‡	17	−1.8
gi\|108877064\|	Low Density Lipoprotein Receptor	18	−11.3*
gi\|157113327\|	Putative D7^‡	19	−3.5
gi\|18568322\|	Putative 30kD Secreted Protein; ‘Short-Form Aegyptin’	20	−14.1*
gi\|157110207\|	Antigen-5/Venom Allergen^‡	21	−2.5
gi\|18568318\|	Putative C-Type Lectin^‡	22	N/A¹
gi\|18568318\|	Putative C-Type Lectin^‡	23	N/A¹

*denotes significance at the p ≤ 0.05 level via Student’s t-test of the mean density of each spot between experimental conditions (n = 2 per condition).
¹quantity at these spots were unable to be calculated due to the location of the bromophenol blue dye front in two of the four gels.
^‡denotes proteins from spots chosen for mass spectrometry identification for the purposes of landmarking.

ISSN: 1756-3305