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Table 1 S. japonicum protein array antigen recognition by rat ASC-probes

From: Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach

  

Recognition* by ASC-probes

 

Name

Accession no.

Skin (No.)

Lung (No.)

Novel∆

Expression profile#

SignalP†

TM∞

Tetraspanin-2 (SjTSP2)

EF553319.1

++

(3)

+++

(4)

No

S

No

Yes

Hypothetical protein

AY815838

+

(1)

+++

(4)

Yes

S

Yes

Yes

22.6 kDa antigen (Sj22.6)

L08198

-

(0)

+++

(3)

No

S, A

No

Yes

Calponin-like

AY813467

-

(0)

++

(3)

Yes

S

No

No

Filamin

XM_002571418

-

(0)

+++

(2)

No

C, S, A, Sp

No

Yes

21.7 kDa antigen (Sj21.7)

AF048759

+

(1)

++

(4)

No

S,A

No

No

Dynein light chain

AF072327.1

-

(0)

++

(2)

No

E, S

No

No

Zinc finger protein

AY223099

++

(1)

+

(2)

Yes

E, A

No

No

Ly6-like (Sj-L6L-1)

AY816003

+

(1)

++

(1)

Yes

S

Yes

Yes

Tetraspanin-3 SmTSP3 (S. mansoni)

CABG01000023

+

(3)

-

(0)

Yes

^

No

Yes

29 kDa antigen (Sj29)

DQ873812.1

+

(3)

+

(1)

No

S, A

Yes

Yes

  1. *Recognition level score: the average signal intensity of the individual(s) which were greater than the mean + 2SD of control serum. High (+++) >10000 SI; Moderate (++) 500–9999 SI; Low (+) 0–499 SI; Negative (-) 0 SI. Number of ASC-probe samples (out of four) that recognised each antigen is indicated in parentheses. ∆Novel: defined as not having been tested as a vaccine candidate against S. japonicum and limited characterisation. #Expression profile: The stage(s) with the highest expression is indicated, based on EST Profile Viewer, UniGene, NCBI: cercariae (C), schistosomula (S), adult worm (A), egg (E), sporocyst (Sp). †Signal peptide determined by SignalP -4.1 (http://www.cbs.dtu.dk/services/SignalP/). ∞Transmembrane prediction by TMpred (http://www.ch.embnet.org/software/TMPRED_form.html) ^Data on sequence not present in UniGene. Three antigens possessing ideal characteristics as novel vaccine candidates are highlighted in bold.