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Table 1 S. japonicum protein array antigen recognition by rat ASC-probes

From: Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach

   Recognition* by ASC-probes  
Name Accession no. Skin (No.) Lung (No.) Novel Expression profile# SignalP† TM∞
Tetraspanin-2 (SjTSP2) EF553319.1 ++ (3) +++ (4) No S No Yes
Hypothetical protein AY815838 + (1) +++ (4) Yes S Yes Yes
22.6 kDa antigen (Sj22.6) L08198 - (0) +++ (3) No S, A No Yes
Calponin-like AY813467 - (0) ++ (3) Yes S No No
Filamin XM_002571418 - (0) +++ (2) No C, S, A, Sp No Yes
21.7 kDa antigen (Sj21.7) AF048759 + (1) ++ (4) No S,A No No
Dynein light chain AF072327.1 - (0) ++ (2) No E, S No No
Zinc finger protein AY223099 ++ (1) + (2) Yes E, A No No
Ly6-like (Sj-L6L-1) AY816003 + (1) ++ (1) Yes S Yes Yes
Tetraspanin-3 SmTSP3 (S. mansoni) CABG01000023 + (3) - (0) Yes ^ No Yes
29 kDa antigen (Sj29) DQ873812.1 + (3) + (1) No S, A Yes Yes
  1. *Recognition level score: the average signal intensity of the individual(s) which were greater than the mean + 2SD of control serum. High (+++) >10000 SI; Moderate (++) 500–9999 SI; Low (+) 0–499 SI; Negative (-) 0 SI. Number of ASC-probe samples (out of four) that recognised each antigen is indicated in parentheses. Novel: defined as not having been tested as a vaccine candidate against S. japonicum and limited characterisation. #Expression profile: The stage(s) with the highest expression is indicated, based on EST Profile Viewer, UniGene, NCBI: cercariae (C), schistosomula (S), adult worm (A), egg (E), sporocyst (Sp). †Signal peptide determined by SignalP -4.1 (http://www.cbs.dtu.dk/services/SignalP/). ∞Transmembrane prediction by TMpred (http://www.ch.embnet.org/software/TMPRED_form.html) ^Data on sequence not present in UniGene. Three antigens possessing ideal characteristics as novel vaccine candidates are highlighted in bold.