Table 1 Primers used in present study
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
Aalb_CTL1 ORF cloning | |||
Aalb_CTL1 | ATGGGGCAGCCATCATCATCATCAT | ACATTCGTTCGCACACAAAAC | 462 |
Recombinant expression | |||
Aalb_CTL1 | GTCCATATG CAACAGGAATGCGACTGTAAAAATAG | GTGAAGCTT ACATTCGTTCGCACACAAAAC | 405 |
Quantitative real-time PCR (Q-PCR) | |||
Aalb_CTL1 | GCTATCTCTCTCGCAGACCTCAC | CCAATCTCCAGCCGTTCCT | 130 |
Rsp 5 | ATTACATCGCCGTCAAGGAG | TCATCATCAGCGAGTTGGTC | 126 |