Treatment | Physiological and biochemical response (adult) | ||||
---|---|---|---|---|---|
Survival rate (%) | Super-cooling point (°C) | Water (%) | Glycerol (μg/mL) | Protein (μg/mL) | |
Control | 100 ± 0.0 | -19.0 ± 3.7 | 56.7 ± 0.6 | 1.1 ± 0.1 | 29.8 ± 2.3 |
0°C 2 h | 100 ± 0.0 | -19.8 ± 2. 7** | 63.3 ± 1.4** | 1.0 ± 0.1 | 11.8 ± 1.7* |
5°C 2 h | 100 ± 0.0 | -20.3 ± 2.4** | 62.2 ± 2.1** | 1.1 ± 0.2 | 10.8 ± 4.8* |
0°C 10d | 98.2 ± 0.7 | -20.7 ± 2.3** | 49.6 ± 3.4** | 3.1 ± 0.4** | 59.3 ± 9.8** |
0°C 20d | 96.5 ± 1.2 | -21.3 ± 1.8** | 48.4 ± 1.0** | 4.4 ± 0.6** | 20.0 ± 4.8 |