Figure 3

Sb(III) susceptibility of clonal lines from L. braziliensis (A) and L. infantum (B) untransfected or transfected with constructs pIR1-BSD or pIR1-BSD-LbcTXNPx. Parasites were incubated in M199 medium in the absence or presence of various concentrations of Sb(III) (0.0125 to 1 mg/ml) for 48 hours and the percentages of live parasites were determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistically different values are indicated as follows: *p < 0.04, **p < 0.007 and ***p < 0.002.