Figure 4
In vitro tolerance of L. braziliensis (A) and L. infantum (B) lines untransfected or transfected with constructs pIR1-BSD/pIR1BSD-cTXNPx, to exogenous hydrogen peroxide. Parasites were cultured for 48 h in the presence of different concentrations of hydrogen peroxide and the percentages of live parasites were determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistically different values are indicated as follows: *p < 0.04, **p < 0.005 and ***p < 0.0001.