Confocal Laser Scanning Microscopy analysis showing LD revealed by BODIPY in T. gondii -infected SkMC after 24 and 48 h of interaction. (A) Image by interferential microscopy showing T. gondii- infected SkMC after 24 h. Note the presence of infected myotubes (thick arrows) and uninfected (thin arrows) in the same culture. (B) Uninfected myotubes (thin arrows) practically does not present LD while cells infected with T. gondii (thick arrows) have numerous LD in green. (C) Interferential microscopy showing T. gondii- infected SkMC after 48 h. Note the presence of infected cells (thick arrows). (D) Double staining T. gondii- infected SkMC in red and LD in green (arrows). Note the major concentration of LD mainly in infected SkMC. Fibroblasts in culture present few LD infected or not (arrowhead). All bars = 20 μm.