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Table 3 Kdr allele frequency (in percentage) of Anopheles sinensis mosquitoes among the three sources of mosquitoes used in the bioassay

From: Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements

Study Sample preparation Bioassay   L1014F L1014C Wildtype L1014 Population mutation frequency*
Site Methods Status n (TTT + TTC) (TGT) (TTG) (TTT+TTC+TGT)
Anhui (Huainan county) Female mosquitoes directly collected from the field Alive 44 81.8 14.8 3.4 94.5a
Dead 37 63.5 28.4 8.1
  Female mosquitoes reared from field-collected larvae Alive 55 78.2 19.1 2.7 94.4a
Dead 26 61.5 26.9 11.5
  F1 female from field collected bloodfed females Alive 53 83.0 11.3 5.7 91.2b
Dead 27 66.7 18.5 14.8
Hunan (Liuyang county) Female mosquitoes directly Alive 89 79.2 8.4 12.4 84.7a
Collected from the field Dead 16 62.5 6.3 31.3
  Female mosquitoes reared from field-collected larvae Alive 121 78.5 12.0 9.5 88.4b
Dead 25 64.0 14.0 22.0
  F1 female from field collected bloodfed females Alive 89 74.2 17.9 7.9 90.5b
Dead 16 56.3 25.0 19.7
Total   Alive 451 78.6 13.5 7.9  
   Dead 147 62.9 21.1 16.0
Laboratory strain   Dead 50 1.0 0.0 99.0 1.0
  1. *For each site, the populations that share different letters indicate statistical difference at P < 0.05 using the analysis of molecular variance.