Table 1 Primers used for cloning of Babesiaorientalis mt genome sequence
Primers | Sequences | Position |
|---|---|---|
P1 | 5′-TGTTAAAAAACTTTATA-3′ | 1-17 |
R1 | 5′-ACTCTATAGGTATTTGACGTAATT-3′ | 1749-1726 |
P2 | 5′-GCATGCAATACCGAACAGGGCCA-3′ | 1592-1617 |
R2 | 5′-GCATTGTCTTATGTAGTTGTTC-3′ | 3246-3225 |
P3 | 5′-AACGACTTCTCTATTGTCTCCAC-3′ | 3368-3390 |
R3 | 5′-CAAATGAGTTATTGGGGAGC-3′ | 5198-5179 |
P4 | 5′-ATAAATTAATTATAACTGTAGCTCC-3′ | 5159-5183 |
R4 | 5′-TGTTAAAAAACTTTATATTTGTTGAAATTT-3′ | 5967-5996 |