Pyrosequencing data of 5 fishborne trematodes. Theoretical pyrogram patterns (top of each panel) and a representative raw data (bottom of each panel) of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis taichui, H. pumilio, and Stellantchasmus falcatus from pyrosequencing. Pyrosequencing based on the large subunit of ribosomal RNA genes was performed by addition of enzyme (E), substrate (S), and four different nucleotides. The letters under the black bars show the dispensation (Disp:) order. The actual sequence detected by pyrosequencing is indicated below the panels after “Seq:”. Y-axis represents the level of fluorescence emitted by the incorporation of a nucleotide base and X-axis represents the total number of bases added at that point in time; A, G, C, T, nucleotide bases.