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Figure 4 | Parasites & Vectors

Figure 4

From: Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens

Figure 4

The amplification products from the analytical specificity testing by conventional PCR. Specificity analysis of amplification of approximate 118–119 bp of 28S rRNA product by OVPyro28S_F and OVPyro28S_R primers with Opisthorchis viverrini (1), Clonorchis sinensis (2), Haplorchis taichui (3), H. pumilio (4), Stellantchasmus falcatus (5), Ascaris lumbricoides (6), Strongyloides stercoralis (7), Necator americanus (8), Trichuris trichiura (9), Capillaria philippinensis (10), Taenia spp. (11), Schistosoma mekongi (12), S. japonicum (13), Echinostoma malayanum (14), Fasciola gigantica (15), Paragonimus heterotremus (16), Isospora belli (17), Trichostrongylus spp. (18), Giardia lamblia (19), genomic DNAs of human leukocytes (20), DNA from non-infected fishes (21) and snails tissues (22). M = 100 bp DNA ladder. Arrows indicate approximate 118–119 bp of amplified PCR product.

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