Table 2 Comparative efficacies of microscopy, Sanger DNA sequencing and pyrosequencing assays in the identification and detection of Opisthorchis viverrini , Clonorchis sinensis , Haplorchis taichui and H. pumilio in various samples
| Â | Sample size | Identification by | ||
|---|---|---|---|---|
Samples | Â | Microscopic examination | Sanger DNA sequencing | Pyrosequencing |
Human Stool | 5 | O. viverrini | O. viverrini | O. viverrini |
Cat stool | 5 | C. sinensis | C. sinensis | C. sinensis |
Cercaria from snail | 4 | Opisthorchiid-like | O. viverrini | O. viverrini |
Metacercaria from fish | 2 | Opisthorchiid-like | O. viverrini | O. viverrini |
Metacercaria from fish | 4 | Opisthorchiid-like (n = 1) | H. pumilio | H. pumilio |
|  |  | Haplorchi s-like (n = 3) | H. pumilio (n = 2) | H. pumilio (n = 2) |
|  |  |  | H. taichui (n = 1) | H. taichui (n = 1) |