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Table 2 Comparative efficacies of microscopy, Sanger DNA sequencing and pyrosequencing assays in the identification and detection of Opisthorchis viverrini , Clonorchis sinensis , Haplorchis taichui and H. pumilio in various samples

From: Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens

  Sample size Identification by
Samples   Microscopic examination Sanger DNA sequencing Pyrosequencing
Human Stool 5 O. viverrini O. viverrini O. viverrini
Cat stool 5 C. sinensis C. sinensis C. sinensis
Cercaria from snail 4 Opisthorchiid-like O. viverrini O. viverrini
Metacercaria from fish 2 Opisthorchiid-like O. viverrini O. viverrini
Metacercaria from fish 4 Opisthorchiid-like (n = 1) H. pumilio H. pumilio
   Haplorchi s-like (n = 3) H. pumilio (n = 2) H. pumilio (n = 2)
    H. taichui (n = 1) H. taichui (n = 1)