Follow-up of BALB/c mice infected with 106trypomastigotes of transgenic Dm28c-luc strain. Bioluminescent images were acquired for the same group of mice and one mouse is shown in the ventral (left panel), dorsal (central panel), and lateral (left panel) position after 10 minutes of D-luciferin injection. The same mouse was evaluated after 1 dpi (A); 4 dpi (B), 7 dpi (C), 14 dpi (D), 22 dpi (E), 29 dpi (F). The settings were binning small (small pixels), 10 seconds exposure. For bioluminescent signal quantification, images of mice in the ventral position were evaluated. Subject ROI (square) was used to identify each individual in the image, average background ROI (circle, red dots) measured the background bioluminescent signal which was used to correct the signal in the automatic measurement ROI (red line, circles). Scale bar of bioluminescent image radiance (photons/sec/cm2/sr) of mouse in ventral position.