Figure 5

Follow-up of BALB/c mice infected with 10⁵trypomastigotes of transgenic Dm28c-luc strain. Bioluminescent images were acquired for the same group of mice, three mice are shown in the ventral (left panel), dorsal (central panel), and lateral (left panel) position after 10 minutes of D-luciferin inoculation. The same three mice were evaluated one hour post infection 0 dpi (A); 1 dpi (B), 7 dpi (C), 14 dpi (D), 21 dpi (E), 28 dpi (F). The settings were binning medium (medium size pixels), 1 minute exposure. For bioluminescent quantification images of mice in the ventral position were evaluated. Subject ROI (square) was used to identify each individual in the image, average background ROI (circle, red dots) measured the background bioluminescent signal which was used to correct the signal in the automatic measurement ROI (red line, circles). Arrows indicate urogenital region. Scale bar of bioluminescent image radiance (photons/ sec/ cm²/sr) of mice in ventral position.