Volume 7 Supplement 1

Proceedings of the 1st Conference on Neglected Vectors and Vector-Borne Diseases (EurNegVec): with Management Committee and Working Group Meetings of the COST Action TD1303

Open Access

Prevalence of Anaplasma phagocytophilum in ticks collected from migratory birds in Danube Delta, Romania

  • ID Mărcuţan1Email author,
  • AD Sándor1,
  • AD Mihalca1,
  • CM Gherman1,
  • Z Kalmár1,
  • G D'Amico1,
  • MO Dumitrache1 and
  • V Cozma1
Parasites & Vectors20147(Suppl 1):P16

https://doi.org/10.1186/1756-3305-7-S1-P16

Published: 1 April 2014

Wild birds are hosts for several species of ticks, contributing to the maintenance of their local populations in delimited geographic areas. migratory birds play important roles as distributors of ticks within and between distant territories, including continents. Ticks collected from birds are responsible for hosting a significant number of human pathogens. The extensive wetland complex of the Danube Delta provides an internationally important stopover site for millions of birds, belonging to 300 different species, travelling annually. The aim of this study was to detect Anaplasma phagocytophilum in feeding ticks collected from migratory birds along four migratory seasons. Ticks were collected from the birds with forceps and preserved in 96% ethanol for later examination using a separate vial for each bird. A total of 1436 birds in 56 species (15 families) of Passeriformes and 3 non-Passeriformes bird species were captured. A total of 400 ticks were collected and identified as larvae (n = 191; 47.75%), nymphs (n = 201; 50.25%) or adult females (n = 8; 2%). No adult males were found. The ticks belonged to four species (Ixodes ricinus, I. arboricola, I. redikorzevi and Haemaphysalis punctata).

Ixodes ricinus was the most common tick (369/400 ticks, 92.25% of the total collected), with a total of 181 larvae, 180 nymphs and 8 females. For detection of Anaplasma phagocytophilum all 400 ticks were examined by PCR, targeting the msp2 gene. Anaplasma phagocytophilum specific DNA was detected in 2 larvae and 2 nymphs of, I. ricinus, from 3 birds prevalence 1.08-%. The infection was absent from the other species of collected ticks

The bird species that carried ticks infected with A. phagocytophilum were Turdus merula, Erithacus rubecula and Fringilla coelebs. Several other studies have demonstrated the presence of A. phagocytophilum in ticks collected from birds. Comparing our data with these results, we confirm the low prevalence of A. phagocytophilum, showingthat migratory birds, despite being without reservoir competencemight be important hosts for the dispersal of infected ticks.

Funding

This research was supported by grant CNCSIS IDEI PCCE 7/2010 and IDEI PCE 236/2011.

Authors’ Affiliations

(1)
Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine

Copyright

© Mărcuţan et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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