`Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

Table 1 Results of nucleotide sequence analysis corresponding to PCR products amplified from the 8 Ctenocephalides felis specimens of this study

Gene*	Flea gender¹	Location	Length of sequence (bp)	% identity	Bacteria with closest identity in the BLAST search	GenBank accession no.
16S rRNA	1/2 M; 6/6 F	Pastaza	1303-1373	99.6-99.9	`Candidatus Rickettsia asemboensis’ (Rickettsia F30)	JN315967
	1/2 M; 0/6 F	Pastaza	1387	97.1	Uncultured flea-associated bacterium	EU137419
	1/2 M; 0/6 F	Pastaza	1387	93.6	Snodgrasella alvi	JQ746645
gltA	2/2 M; 6/6 F	Pastaza	350	100	Rickettsia felis-like (Rickettsia RF2125)	AF516333
gltA	2/2 M; 6/6 F	Pastaza	350	99.7	`Candidatus Rickettsia asemboensis’ (Rickettsia F30)	JN315968
htrA	2/2 M; 6/6 F	Pastaza	394	100	`Candidatus Rickettsia asemboensis’ (Rickettsia F30)/Rickettsia felis-like (Rickettsia SE313/cf1and5)	JN315969/DQ166937/AY953286
ompB	2/2 M; 6/6 F	Pastaza	464	100	`Candidatus Rickettsia asemboensis’ (Rickettsia F30)	JN315972
sca4	2/2 M; 6/6 F	Pastaza	352	100	`Candidatus Rickettsia asemboensis’ (Rickettsia F30)	JN315970

*Inconclusive sequences were obtained for ompA gene.
¹Number of positive specimens / Total number of specimens from each flea gender; M: Male; F: Female.

ISSN: 1756-3305