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Table 1 Results of nucleotide sequence analysis corresponding to PCR products amplified from the 8 Ctenocephalides felis specimens of this study

From: `Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

Gene* Flea gender1 Location Length of sequence (bp) % identity Bacteria with closest identity in the BLAST search GenBank accession no.
16S rRNA 1/2 M; 6/6 F Pastaza 1303-1373 99.6-99.9 `Candidatus Rickettsia asemboensis’ (Rickettsia F30) JN315967
1/2 M; 0/6 F Pastaza 1387 97.1 Uncultured flea-associated bacterium EU137419
93.6 Snodgrasella alvi JQ746645
gltA 2/2 M; 6/6 F Pastaza 350 100 Rickettsia felis-like (Rickettsia RF2125) AF516333
99.7 `Candidatus Rickettsia asemboensis’ (Rickettsia F30) JN315968
htrA 2/2 M; 6/6 F Pastaza 394 100 `Candidatus Rickettsia asemboensis’ (Rickettsia F30)/Rickettsia felis-like (Rickettsia SE313/cf1and5) JN315969/DQ166937/AY953286
ompB 2/2 M; 6/6 F Pastaza 464 100 `Candidatus Rickettsia asemboensis’ (Rickettsia F30) JN315972
sca4 2/2 M; 6/6 F Pastaza 352 100 `Candidatus Rickettsia asemboensis’ (Rickettsia F30) JN315970
  1. *Inconclusive sequences were obtained for ompA gene.
  2. 1Number of positive specimens / Total number of specimens from each flea gender; M: Male; F: Female.