Figure 2

TcNTPDase-1 expression in infective and non-infective forms of T. cruzi . A. TcNTPDase-1 mRNA levels in epimastigotes, amastigotes and trypomastigotes, estimated by RT-qPCR. The relative quantification by ΔΔCt method was performed using the epimastigote form as calibrator. B. Ecto-ATPase and ecto-ADPase activities in distinct developmental forms. The ecto-nucleotidase activities were estimated in epimastigotes, amastigotes and trypomastigotes, using 5 mM ATP or ADP as substrate.*, #p < 0.05 (versus epimastigotes, Mann-Whitney Rank-Sum test).