Figure 1

A very simple method for inducing egg-laying in wild Anopheles funestus that consistently results in >70% of the females ovipositing. Glass vials, 45 mm high x 25 mm diameter, with gauze lids are used. Small pieces of filter paper are placed at an angle in the bottom of the tube with approximately 1 ml water for egg laying. Females, resting inside the plastic lids, can be transferred to clean vials once they have laid eggs, facilitating blood-feeding for multiple egg batches.