Figure 4

Expression of miR-281 in C6/36 cells after transfection with mimics and antagomiRs. (A) The relative expression of miR-281 increases significantly at 24 hrs post-transfection with the miR-281 mimic. (B) The relative miR-281 expression level is significantly reduced by transfection with the miR-281 antagomiRs at 24 hrs post-transfection. mock: normal cells; control mimic: cells transfected with control mimic; miR-281 mimic: cells transfected with miR-281 mimics; control antagomiR: cells transfected with control antagomiRs; antagomiR-281: cells transfected with miR-281 antagomiRs. The relative expression of miR-281 was calculated normalized to 5S rRNA. The RT-qPCR data were analyzed using the 2^-Δct method. Data shown represent three independent experiments with three triplicates each. P values were calculated using ANOVA.