Table 1 Primers and conditions for PCR detection of pathogen DNA
From: Detection and characterization of zoonotic pathogens of free-ranging non-human primates from Zambia
Organism | Target gene | Primer | Sequence (5' to 3') | Amplicon | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|---|
size (bp) | ||||||
Rickettsia spp | gltA | RpCS.780p | GACCATGAGCAGAATGCTTCT | 600 | 48 | [8] |
| Â | Â | RpCS.877p | GGGGACCTGCTCACGGCGG | 480 | 54 | Â |
| Â | Â | RpCS.1273r | CATAACCAGTGTAAAGCTG | Â | Â | Â |
Anaplasma sp.. | 16S rDNA | EHR16SD | GGTACCYACAGAAGAAGTCC | 345 | 53 | [9] |
| Â | Â | EHR16SR | TAGCACTCATCGTTTACAGC | Â | Â | Â |
Coxiella burnetii | IS1111 | Trans 1 | TATGTATCCACCGTAGCCAGTC | 687 | 60 | [10] |
| Â | Â | Trans 2 | CCCAACAACACCTCCTTATTC | Â | Â | Â |
Borrelia spp. | fla gene | BflaPAD | GATCA(G/A)GC(T/A)CAA(C/T)ATAACCA(A/T)ATGCA | Â | 55 | [11] |
| Â | Â | BflaPDU | AGATTCAAGTCTGTTTTGGAAAGC | Â | Â | Â |
| Â | Â | BflaPBU,nest | GCTGAAGAGCTTGGAATGCAACC | 340 | 55 | Â |
| Â | Â | BflaPCR,nest | TGATCAGTTATCATTCTAATAGCA | Â | Â | Â |
B. microti | 18S rDNA | Babl | CTTAGTATAAGCTTTTATACAGC | 238 | 55 | [12] |
| Â | Â | Bab4 | ATAGGTCAGAAACTTGAATGATACA | Â | Â | Â |
Trypanosoma spp. | ITS1 rDNA | ITS1 CF | CCGGAAGTTCACCGATATTG | Variable | 58 | [13] |
ITS1 BR | TTGCTGCGTTCTTCAACGAA | |||||
Leishmania spp. | kDNA minicircle | L.MC-1S | CTRGGGGTTGGTGTAAAATAG- | 700 | 55 | [14] |
| Â | Â | L.MC-1R | TWTGAACGGGRTTTCTG | Â | Â | Â |
Plasmodium spp. | Cytb | DW2 &DW4 | DW2; TAATGCCTAGACGTATTCCTGATTATCCAG | 1253 | 60 | [15] |
DW4; TGTTTGCTTGGGAGCTGTAATCATAATGTG | ||||||
| Â | Â | Cytb1 & Cytb2 | CYTb1; CTCTATTAATTTAGTTAAAGCACA | 939 | 50 | Â |
Tb2; ACAGAATAATCTCTAGCACC |