Figure 2

Neighbor joining trees showing the relationship of Haemonchus contortus phosphoinositide 3-kinase (PI3K) catalytic subunit Hc -AGE-1 and regulatory subunit Hc -AAP-1. The trees were calculated using the Jones-Taylor-Thornton (JTT) model in the MEGA program version 5.0. Bootstrap values above or below the branches (1,000 iterations) are shown for robust clades (>50 %). (a) The species used in the analysis include six nematodes (Strongyloides stercoralis, Ss-AGE-1; Parastrongyloides trichosuri, Pt-AGE-1; Brugia malayi, Bm-AGE-1; Loa loa, Ll-AGE-1; Caenorhabditis elegans, Ce-AGE-1, Ce-PIKII and Ce-VPS34; C. briggsae, Cb-AGE-1) and three non-nematodes (Drosophila melanogaster, Dm-PI3K92E and Dm-PI3K59F; Homo sapiens, Hs-PI3KCA, Hs-PI3KC2 and Hs-PI3KC3; Saccharomyces cerevisiae, Sc-VPS34). Mus musculus EGFR AAA17899 was used as an outgroup. (b) AAP-1 used in the analysis included Caenorhabditis elegans, Ce-AAP-1; C. briggsae, Cb-AAP-1; C. remani, Cr-AAP-1; Strongyloides stercoralis, Ss-AAP-1; Ascaris sum, As-AAP-1; Brugia malayi, Bm-AAP-1; Loa loa, Ll-AAP-1; Trichinella spiralis, Ts-AAP-1; Drosophila melanogaster, Dm-P60; Homo sapiens, Hs-PI3K85). C. elegans Ce-LET-23 CAA93882 was used as an outgroup. GenBank accession numbers are listed on the right of each species.