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Table 1 PCR parameters for amplification of DNA of target organisms

From: Fox on the run – molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria

Target organism Forward primer (5’-3’) No. of cycles Annealing temperature (°C) Primer concentration (pmol) Product size (bp) Reference
Reverse primer (5’-3’)
Anaplasma sp. Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC 30 66.8 12.5 619 [15]
ERB2rev: CTC TTT CGA CCT CTA GTC TAG C
Piroplasms (nested) 1st 40 68 25 561 [16]
BTH-1 F: CCT GAG AAA CGG CTA CCA CAT CT      
BTH-1R: TTG CGA CCA TAC TCC CCC CA      
2nd      
GF2: GTC TTG TAA TTG GAA TGA TGG 40 60 50   
GR2: CCA AAG ACT TTG ATT TCT CTC      
Ehrlichia canis Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC 30 65.0 20 619 [15]
Ehr.CCE.rev: CTC TTT CGA CCT CTA GTC TAG C
Hepatozoon canis HEPF: ATA CAT GAG CAA AAT CTC AAC 35 57.0 10 660 [17]
HEPR: CTT ATT ATT CCA TGC TGC AG
Rickettsia sp. ITS-F: GAT AGG TCG GGT GTG GAA G 35 52 1 342 – 533 [18]
ITS-R: TCG GGA TGG GAT CGT GTG