Table 1 PCR parameters for amplification of DNA of target organisms
Target organism | Forward primer (5’-3’) | No. of cycles | Annealing temperature (°C) | Primer concentration (pmol) | Product size (bp) | Reference |
|---|---|---|---|---|---|---|
Reverse primer (5’-3’) | ||||||
Anaplasma sp. | Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC | 30 | 66.8 | 12.5 | 619 | [15] |
ERB2rev: CTC TTT CGA CCT CTA GTC TAG C | ||||||
Piroplasms (nested) | 1st | 40 | 68 | 25 | 561 | [16] |
BTH-1 F: CCT GAG AAA CGG CTA CCA CAT CT | ||||||
BTH-1R: TTG CGA CCA TAC TCC CCC CA | ||||||
2nd | ||||||
GF2: GTC TTG TAA TTG GAA TGA TGG | 40 | 60 | 50 | |||
GR2: CCA AAG ACT TTG ATT TCT CTC | ||||||
Ehrlichia canis | Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC | 30 | 65.0 | 20 | 619 | [15] |
Ehr.CCE.rev: CTC TTT CGA CCT CTA GTC TAG C | ||||||
Hepatozoon canis | HEPF: ATA CAT GAG CAA AAT CTC AAC | 35 | 57.0 | 10 | 660 | [17] |
HEPR: CTT ATT ATT CCA TGC TGC AG | ||||||
Rickettsia sp. | ITS-F: GAT AGG TCG GGT GTG GAA G | 35 | 52 | 1 | 342 – 533 | [18] |
ITS-R: TCG GGA TGG GAT CGT GTG |