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Table 2 PCR results of spleen and blood compared to sequencing results of the investigated foxes (pos = representing a positive PCR product on the gel, neg = delivering no band on the gel, H.canis or B. microti -like = confirmed sequence of this pathogen in the substrate, ”f” indicates false positive samples showing a gel band, but not confirmed during sequencing)

From: Fox on the run – molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria

 

PCR

  

Fox

Piroplasmsnested

H. canis

Pathogens detected

GenBank® accession no

1

B. microti-like

pos. f

B. microti-like

KM115968

2

H. canis

H. canis

H. canis

KM115969

3

H. canis

pos.

H. canis

KM115970

4

H. canis

H. canis

H. canis

KM115971

5

B. microti-like

neg.

B. microti-like

KM115972

6

B. microti-like

pos. f

B. microti-like

KM115973

7

H. canis

H. canis

H. canis

KM115974

8

B. microti-like

neg.

B. microti-like

KM115975

9

B. microti-like

neg.

B. microti-like

KM115976

10

pos

pos. f

unclear

 

11

B. microti-like

pos. f

B. microti-like

KM115977

12

B. microti-like

neg.

B. microti-like

KM115978

13

H. canis

H. canis

H. canis

KM115979

14

B. microti-like

H. canis

B. microti-like/H. canis

KM115980/KM115981

15

B. microti-like/ H. canis

pos.

B. microti-like/H. canis

KM115982/KM115983

16

H. canis

H. canis

H. canis

KM115984

17

B. microti-like

pos. f

B. microti-like

KM115985

18

H. canis

H. canis

H. canis

KM115986

19

H. canis

H. canis

H. canis

KM115987

20

B. microti-like

H. canis

B. microti-like/H. canis

KM115988/KM115989

21

B. microti-like

neg.

B. microti-like

KM115990

22

H. canis

H. canis

H. canis

KM115991

23

B. microti-like

neg.

B. microti-like

KM115992

24

H. canis

H. canis

H. canis

KM115993

25

B. microti-like

neg.

B. microti-like

KM115994

26

H. canis

H. canis

H. canis

KM115995

27

H. canis

H. canis

H .canis

KM115996

28

B. microti-like

pos. f

B. microti-like

KM115997

29

H. canis

H. canis

H. canis

KM115998

30

B. microti-like

H. canis

B. microti-like/H. canis

KM115999/KM116000

31

H. canis

H. canis

H. canis

KM116001

32

pos.

H. canis

H. canis

KM116002

33

H. canis

H. canis

H. canis

KM116003

34

B. microti-like

neg.

B. microti-like

KM116004

35

H. canis

H. canis

H. canis

KM116005

36

B. microti-like

pos. f

B. microti-like

KM116006

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