Figure 1

Unrooted neighbor-joining tree of the ITS-2 region, demonstrating reciprocal monophyly of Varestrongylus eleguneniensis . Unrooted neighbor-joining tree based on HKY distances at the ITS-2 region of the nuclear ribosomal DNA, demonstrating the identity of adult and larval specimens of V. eleguneniensis produced in the current study and those previously attributed to an unknown species of Varestrongylus at high altitudes of North America. Selected sequences shown in this tree represent adults, L1 and L3 of V. eleguneniensis from the current study, L1 ‘Protostrongylid’ from [12] and L1 of ‘Varestrongylus sp.’ from [15]. Sequences at the ITS-2 locus for other genera and species of protostrongylids included those used in the original comparisons by [12]; GenBank accession numbers in Methods). Bootstraps (5,000) values are only shown for branches with over 95% support. Superscript (*) refers to the holotype and (**) to paratypes of V. eleguneniensis deposited at the United States National Parasite Collection (see Table 1).